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MALDI-TOF-MS用于微生物鑒定的影響因素分析

點擊數:17560  時間:2019-03-04 15:00:31  來源: 中國動物檢疫      作者: 徐淑菲等

影響MALDI-TOF-MS微生物鑒定的因素有很多。本研究主要從微生物培養基、培養狀態、菌體前處理、上樣量等方面進行MALDI-TOF-MS操作技術和方法的探討。研究結果顯示:進行MALDI-TOF-MS細菌鑒定,培養時間在4~24 h之間為佳;盡量選擇無色、不含鹽的液體培養基;0.1 μL菌液是最低鑒定菌量;上樣量對結果無影響;對于菌體前處理,除按照標準化前處理程序操作外,也可將菌體加ddH2O制成混懸液進行點靶鑒定,這樣更加簡便。本研究有助于減少MALDI-TOF-MS操作盲目性,提高鑒定質量,利于操作的規范化和簡便化。


Influence Factors Analysis onMALDI-TOF-MS in Microorganism Identification

Various factors could influence the effectof MALDI-TOF-MS in microorganism identification. In this studythe approaches and techniques ofMALDI-TOF-MS from aspects of microbiological mediumbacteria incubation statebacteria pre-treatment and sample loadingquantity were summarized and discussed. According to the research resultsthe period of bacterium incubation of 4 to24 hours was the bestcolorless and salt-free medium was preferablethe lowest ascertainable bacteria solutionwas 0.1 μLthe sample loading quantity had no effect onidentification result. Besidesfor the standard pre-treatment proceduresa handier way was to add ddH2O into the bacteriato  make suspension and conduct targetidentification. This study would contribute to reduce the operational blindnessof MALDI-TOF-MSenhance the identification quality andfacilitate the standardization and simplification of operation.

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