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犬副流感病毒D121004株的分離鑒定及其F基因遺傳進化分析

點擊數:17650  時間:2019-03-04 14:58:58  來源: 中國動物檢疫      作者: 李 超等

為了給犬副流感病毒(CPIV)疫苗研制提供支持,無菌采集有呼吸道癥狀病死犬的腦和肺臟樣品,處理后接種Marc-145細胞,通過RT-PCR、紅細胞吸附試驗,對盲傳3代的細胞培養液進行鑒定,成功分離到1CPIV。該病毒能使Marc-145細胞出現細胞病變,且具有吸附雞紅細胞的能力,將其命名為CPIV-D121004株。對該毒株的F糖蛋白全基因進行克隆及核苷酸同源性分析并構建遺傳進化樹,發現分離株的F基因較為保守,未出現大的遺傳變異。今后需要對分離株的免疫原性進行研究,以探究其作為候選疫苗株的潛力。


Isolation and Identificationof Canine Parainfluenza Virus D121004 Strain

and Sequence Analysis on ItsF Gene

In order to research and develop a vaccineagainst canine parainfluenza virusCPIV),the brains and lungs of dead dogs suspicious of respiratory symptomswere collected asepticallyand then Marc-145 cells were inoculated after treatment to isolateCPIV. RT-PCR and erythrocyte adsorption assay were applied to identify the cellculture medium with three blind passages. The results showed that one strain ofcanine parainfluenza virusCPIV-D121004was successfully isolatedwhich could cause cytopathic changes in Marc-145 cellsand was capable of adsorbing chicken erythrocytes. Cloning and nucleotide homologyanalysis on the whole F glycoprotein gene were carried outand the genetic evolution tree was built.The results showed that the F gene of the isolated strain was relativelyconservativeindicating no major genetic variation. Infuturethe immunogenicity of the isolate should befurther studiedso as to explore the potential to be thevaccine strain.

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