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H5N1和H7N9亞型雙價滅活流感疫苗對鵪鶉及鴿子的免疫原性

點擊數:18412  時間:2019-03-04 14:54:29  來源: 中國動物檢疫      作者: 譚慶輝等

為評價重組流感病毒(H5+H7)二價滅活疫苗(H5N1 Re-8+H7N9 H7-Re1株)對鵪鶉和鴿子的安全性及免疫效果,201712月—20186月,采用哈爾濱維科生物技術開發公司生產的重組流感病毒(H5+H7)二價滅活疫苗,對鵪鶉和肉鴿,在25日齡首免,46日齡二免(鵪鶉和肉鴿的劑量分別為0.3 mL1 mL),觀察禽群臨床表現,定期采集血清測定抗體效價。結果顯示:鵪鶉首免后21 dH5H7亞型HI抗體效價均達到高峰,分別為(7.7±1.3log2和(8.5±1.3log2,抗體合格率大于70%的持續期約為35 d;二免后7 dH5H7亞型抗體效價均達到高峰,效價分別為(9.7±0.9log2和(10.3±0.9log2H5H7亞型抗體合格率大于70%的持續期分別約為147 d175 d。鴿子首免后21 dH5H7抗體效價也均到達高峰,分別為(9.8±0.8log2和(10.4±1.1log2,兩種亞型抗體合格率大于70%的持續期均超過181 d;加強免疫后7 dH5H7抗體效價也均達到高峰,效價分別為(10.2±1.0log2和(10.7±0.9log2189 d后效價分別為(5.4±1.3log2和(6.1±1.5log2,合格率分別為96.7%100%。臨床觀察,未發現該疫苗有嚴重免疫不良反應。結果表明,(H5+H7)二價滅活疫苗對鵪鶉和鴿子安全有效。生產中推薦:鵪鶉25日齡首免,46日齡和6月齡各加強免疫1次,劑量為0.3 mL;鴿子25日齡首免,以后每隔6個月加強免1次,劑量為1 mL


Immunogenicity Study onBivalent Inactivated Vaccines of H5N1 and H7N9

Subtype Influenza in Quailsand Pigeons

In order to evaluate the safety and immuneeffects of the bivalent inactivated vaccineH5N1 Re-8 + H7N9 H7-Re1 strainof recombinant influenza virusH5+H7in quails and pigeonsthe bivalent inactivated vaccines produced by HarbinWeike Biotechnology Development Company were immunized in quails and youngpigeons from December 2017 to June 2018. The dose for a quail/pigeon was 0.3/1mL in first immunization at 25 days of age and enhanced immunization at 46 daysof age respectively. The clinical manifestations of poultry flocks wereobserved and the titers of antibodies were determined by collecting serumsregularly. The results showed that the titers of anti-HI antibodies of H5 and H7subtypes reached peak values 21 days after first immunization in quails with7.7±1.3log2 and8.5±1.3log2 respectively. The duration of antibody eligibility exceeding 70%was about 35 days. The titers of anti-HI antibodies of H5 and H7 subtypesreached peak values 7 days after enhanced immunization with9.7±0.9log2 and10.3±0.9log2respectively. The effect duration ofantibodies against H5 and H7 subtypes exceeding 70% were about 147 and 175 daysrespectively. The titers of H5 and H7 antibodies reached their peaks 21 daysafter first immunization in young pigeons with9.8±0.8log2 and10.4±1.1log2 respectively. The period when thequalification rates of the two subtypes of antibodies exceeded 70% was morethan 181 days. The titers of H5 and H7 antibodies reached their peaks 7 daysafter enhanced immunization with10.2±1.0log2 and10.7±0.9log2respectively. After 189 daysthe titers were5.4±1.3log2 and6.1±1.5log2 respectivelyand the qualification rates were 96.7% and 100% respectively. Noserious side effects were found in clinical observation. The results showedthatH5+H7bivalent inactivated vaccine was safe and effectivefor quails and pigeons. Thereforeit was recommended that quails should be immunized at25 days of ageand they should receive enhanced immunes at46 days and 180 days at the dose of 0.3 mL. The pigeons should receive fistinoculation at 25 days of ageand enhanced immunizations every 6 months with the dose of 1 mL.

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